Frontispiz: Efficient Phage Display with Multiple Distinct Non‐Canonical Amino Acids Using Orthogonal Ribosome‐Mediated Genetic Code Expansion
نویسندگان
چکیده
منابع مشابه
Rationally evolving tRNAPyl for efficient incorporation of noncanonical amino acids
Genetic encoding of noncanonical amino acids (ncAAs) into proteins is a powerful approach to study protein functions. Pyrrolysyl-tRNA synthetase (PylRS), a polyspecific aminoacyl-tRNA synthetase in wide use, has facilitated incorporation of a large number of different ncAAs into proteins to date. To make this process more efficient, we rationally evolved tRNA(Pyl) to create tRNA(Pyl-opt) with s...
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ژورنال
عنوان ژورنال: Angewandte Chemie
سال: 2019
ISSN: 0044-8249,1521-3757
DOI: 10.1002/ange.201983261